| 质粒名称: | pKS diaCas9_sgRNA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74923 |
| 存储实验室: | Per Winge |
|
载体骨架基本信息 |
|
| 载体名称: | pBlueScript KS(+) |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2904 bp |
| 完整质粒大小: | 8577 bp |
| 载体修饰: | 去除氨苄西林耐药基因中的Bsa1位点 |
| 载体类型: | CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LHCF2 promoter |
| 物种来源: | Phaeodactylum tricornutum |
| 插入大小: | 442 bp |
| 基因突变: | -- |
| GenBank编号 | Z24761.1 |
| 启动子 | Cas9 module |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sac1(未破坏) |
| 3' 克隆位点: | Xba1 (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pKS diaCas9_sgRNA was a gift from Per Winge (Addgene plasmid # 74923 ; http://n2t.net/addgene:74923 ; RRID:Addgene_74923) |
| 参考文献部分: | A CRISPR/Cas9 system adapted for gene editing in marine algae. Nymark M, Sharma AK, Sparstad T, Bones AM, Winge P. Sci Rep. 2016 Apr 25;6:24951. doi: 10.1038/srep24951. 10.1038/srep24951 PubMed 27108533 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pKS diaCas9_sgRNA 8576 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a
sgRNA driven by a U6 promoter.
ACCESSION .
VERSION .
KEYWORDS pKS diaCas9_sgRNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8576)
AUTHORS Nymark M, Sharma AK, Sparstad T, Bones AM, Winge P
TITLE A CRISPR/Cas9 system adapted for gene editing in marine algae.
JOURNAL Sci Rep. 2016 Apr 25;6:24951. doi: 10.1038/srep24951.
PUBMED 27108533
REFERENCE 2 (bases 1 to 8576)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8576
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(140..161)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 352..371
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 588..610
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 602..619
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 603..619
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1117..1182
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1189..1209
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1234..5334
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5335..5382
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
misc_RNA 5918..5993
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(6340..6356)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6341..6357)
/label=pBluescriptKS
/note="For pBluescript vector"
promoter complement(6385..6403)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6424..6440)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6424..6440)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6437..6459)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6448..6464
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6472..6502)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6517..6538
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6655..6672)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6826..7414)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6906..6925)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7585..8445)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8208..8227
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8446..8550)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcaa
661 tctcgcctat tcatggtgta taaaagttca acatccaaag ctagaacttt tggaaagaga
721 aagaatatcc gaatagggca cggcgtgccg tattgttgga gtggactagc agaaagtgag
781 gaaggcacag gatgagtttt ctcgagacac atagcttcag cgtcgtgtag gctaggcaga
841 ggtgagtttt ctcgagacat accttcagcg tcgtcttcac tgtcacagtc aactgacagt
901 aatcgttgat ccggagagat tcaaaattca atctgtttgg acctggataa gacacaagag
961 cgacatcctg acatgaacgc cgtaaacagc aaatcctggt tgaacacgta tccttttggg
1021 ggcctccgct acgacgctcg ctccagctgg ggcttcctta ctatacacag cgcgcatatt
1081 tcacggttgc cagaagtcaa gatggcctct agaatggact ataaggacca cgacggagat
1141 tacaaggatc atgatattga ttacaaagac gatgatgata aaatggcccc aaagaagaag
1201 cggaaagtcg gtatacacgg agtcccagca gccgacaaga agtactcgat tggattggac
1261 atcggcacca actctgttgg atgggccgtc atcacggacg agtacaaggt gcccagcaag
1321 aaattcaaag tactgggaaa cactgaccgc cattcgatca agaagaattt gattggagct
1381 ttgctgtttg acagcggcga aacagcggaa gccacgcggc ttaaacgaac cgcgagacga
1441 agatatacgc gacggaagaa ccgtatctgc tatctacaag agattttctc gaatgagatg
1501 gcaaaagtcg atgactcttt ctttcaccga ttggaagaat ccttcctcgt tgaagaggat
1561 aagaaacatg agcggcaccc gatatttgga aacattgtgg acgaggtggc ttaccatgag
1621 aaatacccga ctatttacca cctgcgaaag aaattggtgg actcgacgga caaagcggat
1681 cttcgcctaa tctatttggc gctagcacat atgattaagt ttcgcggcca cttcttgatc
1741 gaaggcgact tgaatccgga caacagtgat gttgacaaac tgtttattca acttgtacaa
1801 acttacaacc agctctttga agaaaatccc attaacgctt cgggagtgga cgcgaaagcc
1861 atcctttctg cccgactcag caagagccga cgcctagaaa atttgattgc gcaacttccc
1921 ggcgagaaaa agaatggact ttttggaaat cttattgctt tgtcgctggg ccttacgccg
1981 aactttaaga gcaacttcga ccttgcggaa gatgccaaat tgcaactgag taaagacaca
2041 tatgacgacg acctcgacaa tcttttggca caaataggag atcagtacgc cgacctcttt
2101 ttggcggcta agaacttgtc cgacgccatt ctgttgtcgg acatccttcg agttaatacg
2161 gagattacta aggcgccctt gagcgcctct atgatcaaaa gatacgacga gcatcaccaa
2221 gacctgaccc tccttaaagc tctcgtccgg caacagctgc ctgagaagta caaagagatt
2281 ttctttgatc aaagcaaaaa cggatacgcg ggctacattg acgggggagc ctcgcaagaa
2341 gagttctaca agtttattaa accgatcctg gaaaagatgg acggcacgga ggaattactc
2401 gtgaaactta accgagagga ccttttgcgt aagcaacgga ctttcgacaa cggatctatt
2461 ccgcaccaaa tccatctcgg agaattgcac gcaattctgc gccggcaaga agatttttat
2521 ccatttctca aggacaaccg ggaaaagatt gagaaaatct taaccttccg cataccctac
2581 tacgtcggcc ctttggccag gggaaattcg agatttgcct ggatgacgcg aaagagtgag
2641 gaaactatta ctccctggaa tttcgaagaa gtggtagaca aaggagcttc cgcgcaatcc
2701 tttatcgagc gcatgaccaa cttcgataag aacctcccga atgagaaagt cctacccaag
2761 cattcgttgc tctacgaata ctttacggtg tataatgagc tgactaaagt caaatacgtt
2821 acagagggaa tgcgaaaacc ggctttcctc tcgggagagc agaaaaaggc catagtcgat
2881 cttctcttta aaacgaaccg taaagttacc gtgaaacaac taaaagagga ctacttcaag
2941 aaaatcgaat gctttgactc cgttgaaatt tccggcgttg aagatcggtt caatgcctcc
3001 ttgggaacat accacgatct cctgaaaatt atcaaagaca aggactttct tgacaatgag
3061 gaaaacgagg acattcttga agatattgtc ttgacgctaa cactttttga ggatcgagag
3121 atgattgagg aacgccttaa aacctatgcg catctgttcg acgacaaagt tatgaagcaa
3181 ttgaagcgcc ggagatatac gggatgggga aggttgagtc ggaaactgat aaatggcata
3241 cgcgataagc agtccggcaa aacaattctc gatttcttga agtccgacgg atttgcgaat
3301 cgaaacttca tgcaactgat ccatgacgat tcgttgacct ttaaagagga cattcaaaaa
3361 gcccaagtat ccggacaagg agattctttg cacgagcata ttgcgaatct tgctggaagt
3421 ccggctatta aaaagggcat tctccagaca gtaaaggtcg tggatgaact cgttaaagtt
3481 atgggccgac acaaacccga gaacatagtc atcgaaatgg cgagagagaa ccagaccacc
3541 caaaagggac agaaaaattc acgcgagcga atgaagcgga ttgaagaggg gatcaaagag
3601 cttggcagcc aaattctgaa agaacacccg gttgaaaaca cccaattgca gaacgagaag
3661 ctctacctgt actatcttca aaatgggcgt gatatgtacg tcgatcaaga attggacatc
3721 aaccggctat ccgattacga tgtggaccat attgttcctc agtcgtttct caaagacgac
3781 tccatagaca ataaggttct aacgagaagc gacaagaacc gcggcaaatc agacaacgtc
3841 ccctccgagg aagtagttaa gaaaatgaag aattactggc gccaattgct gaacgctaag
3901 ctcattaccc aaagaaagtt tgacaatttg acgaaagccg agcgaggcgg gctctcggaa
3961 ctggataagg ccggcttcat taaaagacaa ttggtggaaa cgcgacaaat cacaaagcac
4021 gtcgcacaga ttctcgactc ccgcatgaac actaagtatg acgagaatga caaactaatc
4081 cgggaagtca aagtgattac cttgaagtcc aagctggttt ccgattttcg taaagatttt
4141 cagttttaca aagttcgcga gatcaacaac taccatcacg cgcacgacgc ttacttgaac
4201 gctgtcgtgg gaacggcctt gattaaaaaa taccctaagc tggaaagcga gttcgtttac
4261 ggcgattaca aggtgtatga cgtacgcaaa atgatcgcga agtcagagca ggaaattggc
4321 aaagctactg cgaagtactt cttttatagc aacataatga attttttcaa aacggagatt
4381 accctcgcta acggcgagat tcgcaagcgc cctcttatcg agacaaacgg cgaaacgggg
4441 gagattgtct gggataaagg ccgcgatttt gcgactgtgc ggaaagtctt gtcgatgccg
4501 caagtgaata tagtcaaaaa gacggaggtg caaacaggcg gcttttcgaa agaatctatc
4561 cttcccaaaa ggaactctga taagttgatt gcgcgaaaaa aggattggga ccctaagaag
4621 tatggcggct ttgacagccc gaccgtagca tattctgtcc ttgtggtggc caaagtcgaa
4681 aagggcaaat ccaagaaact gaagagtgtg aaagaattgc ttgggattac gatcatggaa
4741 agatcatctt ttgagaagaa tcccatcgac tttctcgaag ctaaaggcta caaagaagtg
4801 aaaaaggacc tgattatcaa gttgcctaag tactccttgt ttgagctgga aaatggccgt
4861 aagcgaatgc tcgcttctgc cggcgaactc cagaagggaa acgaattggc gctgccctcc
4921 aaatatgtta actttcttta ccttgcctcg cactatgaga agctcaaggg ctcccccgag
4981 gataatgagc aaaaacaact gtttgtagaa cagcataagc actacttgga cgagatcatt
5041 gagcaaatta gcgagttctc caagcgagtc atccttgccg acgctaatct ggacaaagtc
5101 ctgtccgcct acaataagca ccgcgataag ccaatccgag agcaagcgga gaatattatc
5161 cacttgttta cgctcaccaa tcttggagcc cctgccgcct ttaagtactt tgacaccacc
5221 atcgaccgca agaggtacac cagcaccaaa gaagtcttgg acgccaccct cattcaccaa
5281 tcaatcaccg gcttgtacga gacacgcatc gacctgtctc aactcggagg cgacaaaagg
5341 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agtaatctgg atccctacct
5401 cgactttggc tgggacactt tcagtgagga caagaagctc cagaagcgtg ctatcgaact
5461 caaccaggga cgtgcggcac aaatgggcat ccttgctctc atggtgcacg aacagttggg
5521 agtctctatc cttccttaaa aatttaattt tcattagttg cagtcactcc gctttggttt
5581 cacagtcagg aataacacta gctcgtcttc actgcaggtt ggctcggaag ttggtgttga
5641 cggtgagctg gaaattggtt gtcggtcact gctagcgaga agaaaacgga ggacagaagg
5701 aagtgaaact cggttcgttc tcgacagcct cactgtcaat atgctcattt tcaatcctta
5761 gcgcttttaa tgtcgaattg acggtaaatt gaataggatc tataatatct acaaggtact
5821 ttgacacgcc aagtattcat tgttagtcaa caatatttta gagctttata aggtcaaaaa
5881 acaccttcaa agtcgaggag accgagagag ggtctcagtt ttagagctag aaatagcaag
5941 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
6001 tagaaccgct cacccatgct atcgtatgtc atttacattg acaaactgct atgaatgcgt
6061 ttgcgataac ttcagctcgc tgaagcccta gacatttcgt ttgactaaat tgttttccgg
6121 ttgctttact accgaccggt ctgcgggtag catgtcttgg cttttgcgag ttcgagagaa
6181 tttatggaca agtgcgttta cagtttggaa cacgagtgtg agtgagcgga attggctatc
6241 tataacattc ttatttcagg aggctggtga ttagcagaat tttcaaagat ctcgaatcta
6301 aaatcgccat gggctcgacg aacttttctg caagcttatc gataccgtcg acctcgaggg
6361 ggggcccggt accagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc
6421 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
6481 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
6541 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
6601 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
6661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
6721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
6781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
6841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
6901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
6961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
7021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
7081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
7141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
7201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
7261 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
7321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
7381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
7441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
7501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
7561 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
7621 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
7681 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc
7741 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
7801 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
7861 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
7921 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
7981 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
8041 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
8101 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
8161 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
8221 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
8281 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
8341 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
8401 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
8461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
8521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
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